alamarBlue Protocols
Detailed guides that will help you use alamarBlue
QC check for alamarBlue
Method to find out LD50 utilizing alamarBlue
General methodology for measuring cytotoxicity or proliferation utilizing alamarBlue (Spectrophotometry methodology and Fluorescence methodology)
Method for figuring out optimum size of incubation and plating density
Spectrophotometry calculations for utilizing completely different filters
alamarBlue instance experiments
MCM2 Protocol
Detailed information that will help you use MCM2
Rabbit anti-MCM2 Proliferation Pairs Staining Procedure
ELISA Protocols
General ELISA Protocols
Indirect ELISA
Competition (Inhibition) ELISA
Direct ELISA with streptavidin-biotin detection
Sandwich ELISA with streptavidin-biotin detection
Sandwich ELISA with direct detection
Protocols to be used with Anti-biotherapeutic Antibodies
PK bridging ELISA measuring free drug
PK bridging ELISA measuring complete drug
PK antigen seize ELISA measuring certain drug completely
ADA bridging ELISA
Flow Cytometry Protocols
Protocols for detecting cell floor and intracellular markers
Direct immunofluorescence staining of cells and blood for Flow Cytometry
Indirect immunofluorescence staining of cells and blood for Flow Cytometry
Direct Immunofluorescence Staining of Immunoglobulin Light Chains on B Lymphocytes in Whole Blood by Flow Cytometry
Direct staining of intracellular antigens by Flow Cytometry (utilizing LEUCOPERM™)
Direct staining of intracellular antigens by Flow Cytometry: methanol methodology
Direct Staining of Intracellular Cytokines by Flow Cytometry
Preparation of Cells for Flow Cytometry
Whole Blood Protocol for Analysis of Intracellular Cytokines by Flow Cytometry
Digitonin Permeabilisation of Cells for Flow Cytometry
Direct Staining of Intracellular Antigens by Flow Cytometry – the paraformaldehyde/saponin methodology
BrdU staining of cells for cell cycle evaluation
Propidium iodide staining of cells for cell cycle evaluation
Immunofluorescence staining of cells together with PI staining of cells for cell cycle evaluation
BrdU staining of cells for cell cycle evaluation and apoptosis
Unprimed T Cell Activation – Pharmacologic Methods
Unprimed T Cell Activation – Antibody Stimulation Methods
Primed T Cell Activation – Antigen Presenting Cell Co-Culture
Measuring Cell Proliferation Using Cell Permeable Dyes
BrdU staining of cells for cell cycle evaluation with Mouse Anti-BrdU Antibody (Bu20a)
Direct Staining of Intracellular CD68: Leucoperm™ Accessory Reagent Method
BrdU staining of cells for cell cycle evaluation with Rabbit Anti-BrdU Antibody
Immunofluorescence (IF) Protocol
Fluorescence Microscopy (Direct Immunofluorescence) for cell suspensions
BrdU Labeling of HeLa Cells Followed by Immunostaining with Mouse Anti-BrdU Antibody
BrdU Labeling of HeLa Cells Followed by Immunostaining with Rabbit Anti-BrdU Antibody
Immunohistochemistry (IHC) Protocols
Protocols for IHC-frozen and IHC-paraffin
Indirect Immunostaining of Frozen Tissue Sections
Streptavidin-Biotin Immunostaining of Frozen Tissue Sections
PAP/APAAP Immunostaining of Frozen Tissue Sections
Indirect Immunostaining of Paraffin-Embedded Tissue Sections
Streptavidin-Biotin Immunostaining of Paraffin-Embedded Tissue Sections
PAP/APAAP immunostaining of paraffin embedded tissue sections
Antigen Retrieval Techniques to be used with Formalin-Fixed Paraffin-Embedded Sections
Antigen Retrieval Techniques: For use with F4/80 Immunostaining of Paraffin-Embedded Sections
Fixative Recipes
Immunoprecipitation (IP) Protocol
Immunoprecipitation with CertainBeads™ Protein A/G Magnetic Beads
Western Blot (WB) Protocol
Western Blotting protocol
Detection of Phosphorylated Proteins by Western Blotting