Systems biology approaches based discovery of a small molecule inhibitor targeting both c-Met/PARP-1 and inducing cell death in breast cancer.

Breast cancer is the second most common types of cancer worldwide. Molecular strategies have developed rapidly; however, novel treatments strategies with high efficacy and lower toxicity are still urgently demanded.

Notably, biological networks estimated from microarraydata and functional activity network analysis could be utilized to identify and validate potential targets. In this study, two microarraydata (GSE13477, GSE31192) were firstly selected, and analyzed by multi-functional activity network analysis to generate the core protein-protein-interaction (PPI) network.

Several potential targets were subsequently identified and c-Met and poly (ADP-ribose) polymerase-1 (PARP-1) were manually chosen as the key targets in breast cancer. Furthermore, virtual screening and molecular dynamics (MD) simulations were utilized to recognize novel c-Met/PARP-1 inhibitors in Specs products database.

Three small molecules, namely, ZINC19909930, ZINC20032678 and ZINC13562414 were selected. Additionally, these compounds were synthesized, and two breast cancer cell lines, MDA-MB-231 and MCF-7 cells were used to validate our bioinformatic findings in vitro. MTT assay and Hoechst staining showed that ZINC20032678 significantly induced breast cancer cell death, which was mediated through apoptosis by flow cytometry.

Furthermore, ZINC20032678 was shown to target the active sites of the both targets and recruitment of downstream apoptotic signaling pathways, eventually inducing breast cancer cell apoptosis.

AES Antibody

36095-100ul 100ul
EUR 252

AES Antibody

1-CSB-PA001405ESR2HU
  • EUR 222.00
  • EUR 335.00
  • 100ul
  • 50ul
  • Form: Liquid
  • Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. Antigen Affinity Purified
Description: A polyclonal antibody against AES. Recognizes AES from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200

AES Antibody

1-CSB-PA973928
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
  • Form: Liquid
  • Buffer: -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol Antigen affinity purification
Description: A polyclonal antibody against AES. Recognizes AES from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:25-1:100

AES Antibody

1-CSB-PA551903
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
  • Form: Liquid
  • Buffer: -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol Antigen affinity purification
Description: A polyclonal antibody against AES. Recognizes AES from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:25-1:100

AES antibody

70R-7912 50 ug
EUR 467
Description: Affinity purified rabbit polyclonal AES antibody

AES siRNA

20-abx900213
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.

AES siRNA

20-abx906940
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.

AES siRNA

20-abx906941
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.

AES Protein

20-abx262697
  • EUR 328.00
  • EUR 6397.00
  • EUR 230.00
  • 10 ug
  • 1 mg
  • 2 µg
  • Shipped within 5-10 working days.

AES Blocking Peptide

33R-3414 100 ug
EUR 180
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of AES antibody, catalog no. 70R-1059

AES Blocking Peptide

33R-3767 100 ug
EUR 180
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of AES antibody, catalog no. 70R-7912

Polyclonal AES Antibody

AMM08746G 0.1 mg
EUR 659
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human AES . This antibody is tested and proven to work in the following applications:

Polyclonal AES Antibody

AMM08747G 0.1 mg
EUR 659
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human AES . This antibody is tested and proven to work in the following applications:

Anti-AES Antibody

A01757-1 100ug/vial
EUR 334

AES Conjugated Antibody

C36095 100ul
EUR 397

AES Rabbit pAb

A7874-100ul 100 ul
EUR 308

AES Rabbit pAb

A7874-200ul 200 ul
EUR 459

AES Rabbit pAb

A7874-20ul 20 ul
EUR 183

AES Rabbit pAb

A7874-50ul 50 ul
EUR 223

AES Polyclonal Antibody

ABP57720-003ml 0.03ml
EUR 158
  • Immunogen information: Synthesized peptide derived from part region of human AES protein
  • Applications tips:
Description: A polyclonal antibody for detection of AES from Human, Mouse, Rat. This AES antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human AES protein

AES Polyclonal Antibody

ABP57720-01ml 0.1ml
EUR 289
  • Immunogen information: Synthesized peptide derived from part region of human AES protein
  • Applications tips:
Description: A polyclonal antibody for detection of AES from Human, Mouse, Rat. This AES antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human AES protein

AES Polyclonal Antibody

ABP57720-02ml 0.2ml
EUR 414
  • Immunogen information: Synthesized peptide derived from part region of human AES protein
  • Applications tips:
Description: A polyclonal antibody for detection of AES from Human, Mouse, Rat. This AES antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human AES protein

AES Polyclonal Antibody

ES10992-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against AES from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA

AES Polyclonal Antibody

ES10992-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against AES from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA

Anti-AES antibody

STJ110184 100 µl
EUR 277
Description: The protein encoded by this gene is similar in sequence to the amino terminus of Drosophila enhancer of split groucho, a protein involved in neurogenesis during embryonic development. The encoded protein, which belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes. Three transcript variants encoding different isoforms have been found for this gene.

Anti-AES antibody

STJ11100820 100 µl
EUR 413
Description: The protein encoded by this gene is similar in sequence to the amino terminus of Drosophila enhancer of split groucho, a protein involved in neurogenesis during embryonic development. The encoded protein, which belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes. Three transcript variants encoding different isoforms have been found for this gene.

Anti-AES antibody

STJ72590 100 µg
EUR 359

Anti-AES antibody

STJ192150 200 µl
EUR 197
Description: Unconjugated Rabbit polyclonal to AES

AES protein (His tag)

80R-1712 50 ug
EUR 305
Description: Purified recombinant Human AES protein

AES ELISA KIT|Human

EF004609 96 Tests
EUR 689

Rat AES shRNA Plasmid

20-abx985519
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg
  • Shipped within 15-20 working days.

Human AES shRNA Plasmid

20-abx950120
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg
  • Shipped within 15-20 working days.

Mouse AES shRNA Plasmid

20-abx970657
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg
  • Shipped within 15-20 working days.

AES Recombinant Protein (Human)

RP036493 100 ug Ask for price

AES Recombinant Protein (Mouse)

RP114653 100 ug Ask for price

AES Recombinant Protein (Rat)

RP189452 100 ug Ask for price

Butyrylcholinesterase

44-0120 1 kU
EUR 259
Description: Butyrylcholine Esterase enzyme

Polyclonal AES Antibody (C-term)

APR14860G 0.1ml
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human AES (C-term). This antibody is tested and proven to work in the following applications:

Polyclonal AES Antibody (N-Term)

APR14862G 0.1 mg
EUR 484
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human AES (N-Term). This antibody is tested and proven to work in the following applications:

[KO Validated] AES Rabbit pAb

A19944-100ul 100 ul
EUR 410

[KO Validated] AES Rabbit pAb

A19944-200ul 200 ul
EUR 571

[KO Validated] AES Rabbit pAb

A19944-20ul 20 ul
EUR 221

[KO Validated] AES Rabbit pAb

A19944-50ul 50 ul
EUR 287

Aes ORF Vector (Rat) (pORF)

ORF063152 1.0 ug DNA
EUR 506

AES ORF Vector (Human) (pORF)

ORF012165 1.0 ug DNA
EUR 354

Aes ORF Vector (Mouse) (pORF)

ORF038219 1.0 ug DNA
EUR 506

Polyclonal AES / Groucho Antibody (C-Terminus)

AMM08744G 0.05mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human AES / Groucho (C-Terminus). This antibody is tested and proven to work in the following applications:

Polyclonal AES / Groucho Antibody (N-Terminus)

AMM08745G 0.05mg
EUR 484
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human AES / Groucho (N-Terminus). This antibody is tested and proven to work in the following applications:

Polyclonal AES antibody - C-terminal region

AMM08748G 0.1mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human AES - C-terminal region. This antibody is tested and proven to work in the following applications:

Polyclonal Aes Antibody - N-terminal region

AMM08749G 0.05mg
EUR 528
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Aes - N-terminal region. This antibody is tested and proven to work in the following applications:

Aes sgRNA CRISPR Lentivector set (Rat)

K6789301 3 x 1.0 ug
EUR 339

AES sgRNA CRISPR Lentivector set (Human)

K0054101 3 x 1.0 ug
EUR 339

Aes sgRNA CRISPR Lentivector set (Mouse)

K4753001 3 x 1.0 ug
EUR 339

Human Amino-terminal enhancer of split (AES)

1-CSB-EP001405HU
  • EUR 380.00
  • EUR 214.00
  • EUR 1309.00
  • EUR 560.00
  • EUR 873.00
  • EUR 262.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 56.1 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Human Amino-terminal enhancer of split(AES) expressed in E.coli

Amino-Terminal Enhancer of Split (AES) Antibody

20-abx007180
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
  • Shipped within 5-10 working days.

Amino-Terminal Enhancer of Split (AES) Antibody

abx027807-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.

Amino-Terminal Enhancer of Split (AES) Antibody

abx027807-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.

Amino-terminal enhancer of split (AES) Antibody

20-abx210483
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul
  • Shipped within 5-10 working days.

Amino-terminal enhancer of split (AES) Antibody

20-abx210484
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul
  • Shipped within 5-10 working days.

Monoclonal AES Antibody (monoclonal) (M02), Clone: 10

APR14861G 0.1mg
EUR 484
Description: A Monoclonal antibody against Human AES (monoclonal) (M02). The antibodies are raised in mouse and are from clone 10. This antibody is applicable in E

Rabbit Anti Aes (C-Terminal) Polyclonal Antibody

CPBT-66226RA 50 µg
EUR 559

Rabbit Anti Aes (C-Terminal) Polyclonal Antibody

CPBT-66227RA 0.1 mg
EUR 580

Amino-Terminal Enhancer of Split (AES) Antibody

20-abx320627
  • EUR 300.00
  • EUR 244.00
  • 100 ul
  • 50 ul
  • Shipped within 5-10 working days.

Amino-Terminal Enhancer of Split (AES) Antibody

abx431308-200ul 200 ul
EUR 384
  • Shipped within 1-3 working days.

Amino-Terminal Enhancer of Split (AES) Antibody

20-abx225017
  • EUR 370.00
  • EUR 606.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 50 ul
  • Shipped within 5-10 working days.

Aes sgRNA CRISPR Lentivector (Rat) (Target 1)

K6789302 1.0 ug DNA
EUR 154

Aes sgRNA CRISPR Lentivector (Rat) (Target 2)

K6789303 1.0 ug DNA
EUR 154

Aes sgRNA CRISPR Lentivector (Rat) (Target 3)

K6789304 1.0 ug DNA
EUR 154

AES sgRNA CRISPR Lentivector (Human) (Target 1)

K0054102 1.0 ug DNA
EUR 154

AES sgRNA CRISPR Lentivector (Human) (Target 2)

K0054103 1.0 ug DNA
EUR 154

AES sgRNA CRISPR Lentivector (Human) (Target 3)

K0054104 1.0 ug DNA
EUR 154

Aes sgRNA CRISPR Lentivector (Mouse) (Target 1)

K4753002 1.0 ug DNA
EUR 154

Aes sgRNA CRISPR Lentivector (Mouse) (Target 2)

K4753003 1.0 ug DNA
EUR 154

Aes sgRNA CRISPR Lentivector (Mouse) (Target 3)

K4753004 1.0 ug DNA
EUR 154

AES Protein Vector (Mouse) (pPB-C-His)

PV152874 500 ng
EUR 603

AES Protein Vector (Mouse) (pPB-N-His)

PV152875 500 ng
EUR 603

AES Protein Vector (Mouse) (pPM-C-HA)

PV152876 500 ng
EUR 603

AES Protein Vector (Mouse) (pPM-C-His)

PV152877 500 ng
EUR 603

AES Protein Vector (Rat) (pPB-C-His)

PV252606 500 ng
EUR 603

AES Protein Vector (Rat) (pPB-N-His)

PV252607 500 ng
EUR 603

AES Protein Vector (Rat) (pPM-C-HA)

PV252608 500 ng
EUR 603

AES Protein Vector (Rat) (pPM-C-His)

PV252609 500 ng
EUR 603

AES Protein Vector (Human) (pPB-His-MBP)

PV320286 500 ng
EUR 481

AES Protein Vector (Human) (pPB-His-GST)

PV320287 500 ng
EUR 481

AES Protein Vector (Human) (pPB-C-His)

PV048657 500 ng
EUR 481

AES Protein Vector (Human) (pPB-N-His)

PV048658 500 ng
EUR 481

AES Protein Vector (Human) (pPM-C-HA)

PV048659 500 ng
EUR 481

AES Protein Vector (Human) (pPM-C-His)

PV048660 500 ng
EUR 481

Aes 3'UTR Luciferase Stable Cell Line

TU101481 1.0 ml Ask for price

Aes 3'UTR GFP Stable Cell Line

TU151481 1.0 ml Ask for price

Aes 3'UTR Luciferase Stable Cell Line

TU200361 1.0 ml Ask for price

Aes 3'UTR GFP Stable Cell Line

TU250361 1.0 ml Ask for price

AES 3'UTR GFP Stable Cell Line

TU050422 1.0 ml
EUR 1394

AES 3'UTR Luciferase Stable Cell Line

TU000422 1.0 ml
EUR 1394

Human basic fibroblast growth factor 6,bFGF-6 ELISA Kit

201-12-0120 96 tests
EUR 440
  • This basic fibroblast growth factor 6 ELISA kit is validated to work with samples from whole blood, serum, plasma and cell culture supernatant.
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Galanthus nivalis lectin (GNA)

05-0120-1000 1 g Ask for price

Collectively, our findings not only offer systems biology approaches based drug target identification, but also provide the new clues for developing novel inhibitors for future breast cancer research.