Systems biology approaches based discovery of a small molecule inhibitor targeting both c-Met/PARP-1 and inducing cell death in breast cancer.

Breast cancer is the second most common types of cancer worldwide. Molecular strategies have developed rapidly; however, novel treatments strategies with high efficacy and lower toxicity are still urgently demanded.

Notably, biological networks estimated from microarraydata and functional activity network analysis could be utilized to identify and validate potential targets. In this study, two microarraydata (GSE13477, GSE31192) were firstly selected, and analyzed by multi-functional activity network analysis to generate the core protein-protein-interaction (PPI) network.

Several potential targets were subsequently identified and c-Met and poly (ADP-ribose) polymerase-1 (PARP-1) were manually chosen as the key targets in breast cancer. Furthermore, virtual screening and molecular dynamics (MD) simulations were utilized to recognize novel c-Met/PARP-1 inhibitors in Specs products database.

Three small molecules, namely, ZINC19909930, ZINC20032678 and ZINC13562414 were selected. Additionally, these compounds were synthesized, and two breast cancer cell lines, MDA-MB-231 and MCF-7 cells were used to validate our bioinformatic findings in vitro. MTT assay and Hoechst staining showed that ZINC20032678 significantly induced breast cancer cell death, which was mediated through apoptosis by flow cytometry.

Furthermore, ZINC20032678 was shown to target the active sites of the both targets and recruitment of downstream apoptotic signaling pathways, eventually inducing breast cancer cell apoptosis.

AES Antibody

1-CSB-PA551903
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against AES. Recognizes AES from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:25-1:100

AES Antibody

1-CSB-PA973928
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against AES. Recognizes AES from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:25-1:100

AES Antibody

1-CSB-PA001405ESR2HU
  • EUR 222.00
  • EUR 335.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against AES. Recognizes AES from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200

AES antibody

70R-7912 50 ug
EUR 467
Description: Affinity purified rabbit polyclonal AES antibody

AES Antibody

3607-002mg 0.02 mg
EUR 171.82
Description: AES Antibody: Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis. It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.

AES Antibody

3607-01mg 0.1 mg
EUR 436.42
Description: AES Antibody: Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis. It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.

AES Antibody

3609-002mg 0.02 mg
EUR 171.82
Description: AES Antibody: Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis. It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.

AES Antibody

3609-01mg 0.1 mg
EUR 436.42
Description: AES Antibody: Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis. It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.

AES antibody

70R-1059 100 ug
EUR 377
Description: Rabbit polyclonal AES antibody raised against the C terminal of AES

AES Peptide

3607P 0.05 mg
EUR 164.75
Description: (CT) AES peptide

AES Peptide

3609P 0.05 mg
EUR 164.75
Description: (NT) AES peptide

AES Peptide

42-365P 0.1 mg
EUR 338
Description: (NT) AES Peptide

AES Polyclonal Antibody

ES10992-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against AES from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA

AES Polyclonal Antibody

ES10992-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against AES from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA

Anti-AES Antibody

A01757-1 100ug/vial
EUR 334

AES Polyclonal Antibody

ABP57720-003ml 0.03ml
EUR 158
Description: A polyclonal antibody for detection of AES from Human, Mouse, Rat. This AES antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human AES protein

AES Polyclonal Antibody

ABP57720-01ml 0.1ml
EUR 289
Description: A polyclonal antibody for detection of AES from Human, Mouse, Rat. This AES antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human AES protein

AES Polyclonal Antibody

ABP57720-02ml 0.2ml
EUR 414
Description: A polyclonal antibody for detection of AES from Human, Mouse, Rat. This AES antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human AES protein

Anti-AES antibody

STJ110184 100 µl
EUR 277
Description: The protein encoded by this gene is similar in sequence to the amino terminus of Drosophila enhancer of split groucho, a protein involved in neurogenesis during embryonic development. The encoded protein, which belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes. Three transcript variants encoding different isoforms have been found for this gene.

Anti-AES antibody

STJ11100820 100 µl
EUR 413
Description: The protein encoded by this gene is similar in sequence to the amino terminus of Drosophila enhancer of split groucho, a protein involved in neurogenesis during embryonic development. The encoded protein, which belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes. Three transcript variants encoding different isoforms have been found for this gene.

Anti-AES antibody

STJ72590 100 µg
EUR 359

Anti-AES antibody

STJ192150 200 µl
EUR 197
Description: Unconjugated Rabbit polyclonal to AES

Polyclonal AES Antibody

AMM08746G 0.1 mg
EUR 659
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human AES . This antibody is tested and proven to work in the following applications:

Polyclonal AES Antibody

AMM08747G 0.1 mg
EUR 659
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human AES . This antibody is tested and proven to work in the following applications:

AES Rabbit pAb

A7874-100ul 100 ul
EUR 308

AES Rabbit pAb

A7874-200ul 200 ul
EUR 459

AES Rabbit pAb

A7874-20ul 20 ul
EUR 183

AES Rabbit pAb

A7874-50ul 50 ul
EUR 223

AES Conjugated Antibody

C36095 100ul
EUR 397

AES Blocking Peptide

33R-3414 100 ug
EUR 180
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of AES antibody, catalog no. 70R-1059

AES Blocking Peptide

33R-3767 100 ug
EUR 180
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of AES antibody, catalog no. 70R-7912

Butyrylcholinesterase

44-0120 1 kU
EUR 259
Description: Butyrylcholine Esterase enzyme

Rat AES shRNA Plasmid

20-abx985519
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

Human AES shRNA Plasmid

20-abx950120
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

Mouse AES shRNA Plasmid

20-abx970657
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

AES ELISA KIT|Human

EF004609 96 Tests
EUR 689

AES Recombinant Protein (Mouse)

RP114653 100 ug Ask for price

AES Recombinant Protein (Rat)

RP189452 100 ug Ask for price

AES Recombinant Protein (Human)

RP036493 100 ug Ask for price

AES protein (His tag)

80R-1712 50 ug
EUR 305
Description: Purified recombinant Human AES protein

[KO Validated] AES Rabbit pAb

A19944-100ul 100 ul
EUR 410

[KO Validated] AES Rabbit pAb

A19944-200ul 200 ul
EUR 571

[KO Validated] AES Rabbit pAb

A19944-20ul 20 ul
EUR 221

[KO Validated] AES Rabbit pAb

A19944-50ul 50 ul
EUR 287

Polyclonal AES Antibody (C-term)

APR14860G 0.1ml
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human AES (C-term). This antibody is tested and proven to work in the following applications:

Polyclonal AES Antibody (N-Term)

APR14862G 0.1 mg
EUR 484
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human AES (N-Term). This antibody is tested and proven to work in the following applications:

AES ORF Vector (Human) (pORF)

ORF012165 1.0 ug DNA
EUR 354

Aes ORF Vector (Mouse) (pORF)

ORF038219 1.0 ug DNA
EUR 506

Aes ORF Vector (Rat) (pORF)

ORF063152 1.0 ug DNA
EUR 506

Polyclonal AES / Groucho Antibody (C-Terminus)

AMM08744G 0.05mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human AES / Groucho (C-Terminus). This antibody is tested and proven to work in the following applications:

Polyclonal AES / Groucho Antibody (N-Terminus)

AMM08745G 0.05mg
EUR 484
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human AES / Groucho (N-Terminus). This antibody is tested and proven to work in the following applications:

Polyclonal AES antibody - C-terminal region

AMM08748G 0.1mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human AES - C-terminal region. This antibody is tested and proven to work in the following applications:

Polyclonal Aes Antibody - N-terminal region

AMM08749G 0.05mg
EUR 528
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Aes - N-terminal region. This antibody is tested and proven to work in the following applications:

AES sgRNA CRISPR Lentivector set (Human)

K0054101 3 x 1.0 ug
EUR 339

Aes sgRNA CRISPR Lentivector set (Mouse)

K4753001 3 x 1.0 ug
EUR 339

Aes sgRNA CRISPR Lentivector set (Rat)

K6789301 3 x 1.0 ug
EUR 339

Rabbit Anti Aes (C-Terminal) Polyclonal Antibody

CPBT-66226RA 50 µg
EUR 559

Rabbit Anti Aes (C-Terminal) Polyclonal Antibody

CPBT-66227RA 0.1 mg
EUR 580

Amino-Terminal Enhancer of Split (AES) Antibody

20-abx007180
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Amino-Terminal Enhancer of Split (AES) Antibody

abx027807-400ul 400 ul
EUR 523

Amino-Terminal Enhancer of Split (AES) Antibody

abx027807-80l 80 µl
EUR 286

Amino-Terminal Enhancer of Split (AES) Antibody

abx431308-200ul 200 ul
EUR 384

Amino-Terminal Enhancer of Split (AES) Antibody

20-abx320627
  • EUR 300.00
  • EUR 244.00
  • 100 ul
  • 50 ul

Amino-Terminal Enhancer of Split (AES) Antibody

20-abx225017
  • EUR 370.00
  • EUR 606.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 50 ul

Amino-terminal enhancer of split (AES) Antibody

20-abx210483
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

Amino-terminal enhancer of split (AES) Antibody

20-abx210484
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

Monoclonal AES Antibody (monoclonal) (M02), Clone: 10

APR14861G 0.1mg
EUR 484
Description: A Monoclonal antibody against Human AES (monoclonal) (M02). The antibodies are raised in mouse and are from clone 10. This antibody is applicable in E

Human Amino-terminal enhancer of split (AES)

1-CSB-EP001405HU
  • EUR 380.00
  • EUR 214.00
  • EUR 1309.00
  • EUR 560.00
  • EUR 873.00
  • EUR 262.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Amino-terminal enhancer of split(AES) expressed in E.coli

AES sgRNA CRISPR Lentivector (Human) (Target 1)

K0054102 1.0 ug DNA
EUR 154

AES sgRNA CRISPR Lentivector (Human) (Target 2)

K0054103 1.0 ug DNA
EUR 154

AES sgRNA CRISPR Lentivector (Human) (Target 3)

K0054104 1.0 ug DNA
EUR 154

Aes sgRNA CRISPR Lentivector (Mouse) (Target 1)

K4753002 1.0 ug DNA
EUR 154

Aes sgRNA CRISPR Lentivector (Mouse) (Target 2)

K4753003 1.0 ug DNA
EUR 154

Aes sgRNA CRISPR Lentivector (Mouse) (Target 3)

K4753004 1.0 ug DNA
EUR 154

Aes sgRNA CRISPR Lentivector (Rat) (Target 1)

K6789302 1.0 ug DNA
EUR 154

Aes sgRNA CRISPR Lentivector (Rat) (Target 2)

K6789303 1.0 ug DNA
EUR 154

Aes sgRNA CRISPR Lentivector (Rat) (Target 3)

K6789304 1.0 ug DNA
EUR 154

AES 3'UTR Luciferase Stable Cell Line

TU000422 1.0 ml
EUR 1394

Aes 3'UTR GFP Stable Cell Line

TU250361 1.0 ml Ask for price

Aes 3'UTR Luciferase Stable Cell Line

TU200361 1.0 ml Ask for price

Aes 3'UTR GFP Stable Cell Line

TU151481 1.0 ml Ask for price

AES 3'UTR GFP Stable Cell Line

TU050422 1.0 ml
EUR 1394

Aes 3'UTR Luciferase Stable Cell Line

TU101481 1.0 ml Ask for price

AES Protein Vector (Human) (pPB-C-His)

PV048657 500 ng
EUR 481

AES Protein Vector (Human) (pPB-N-His)

PV048658 500 ng
EUR 481

AES Protein Vector (Human) (pPM-C-HA)

PV048659 500 ng
EUR 481

AES Protein Vector (Human) (pPM-C-His)

PV048660 500 ng
EUR 481

AES Protein Vector (Human) (pPB-His-MBP)

PV320286 500 ng
EUR 481

AES Protein Vector (Human) (pPB-His-GST)

PV320287 500 ng
EUR 481

AES Protein Vector (Rat) (pPB-C-His)

PV252606 500 ng
EUR 603

AES Protein Vector (Rat) (pPB-N-His)

PV252607 500 ng
EUR 603

AES Protein Vector (Rat) (pPM-C-HA)

PV252608 500 ng
EUR 603

Collectively, our findings not only offer systems biology approaches based drug target identification, but also provide the new clues for developing novel inhibitors for future breast cancer research.